Anti-Glutamate receptor 2 (Precursor) (phospho-Ser880) Antibody

WB

WB: 1:500~1:1000

The antiserum was produced against synthesized phosphopeptide derived from human Glutamate receptor 2 (Precursor) around the phosphorylation site of serine 880 (I-E-SP-V-K).

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

PCR products and double-stranded DNA were digested with appropriate restriction enzymes to completion to yield 19 bands, ranging from 100 pb – 10 kb, for molecular weight standards in agarose gel electrophoresis. The 500, 1,500, and 3,000 base pair bands have increased intensity to serve as quick reference points. Approximated mass of each DNA band is provided (for a loading size of 5 μl of the DNA ladder) for approximating the mass of DNA in comparably intense DNA samples of similar size.