Alkaline Phosphatase Conjugated Affinity Purified anti-Human IgG [H and L] [Rabbit]
| Specifications | |
|---|---|
| Type: | Secondary Antibody |
| Host: | Rabbit |
| Reactivity: | Human |
| Label: | Alkaline Phosphatase |
| Concentration: | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Buffer: | 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Stabilizer: | 10 mg/ml BSA, IgG and Protease free |
| Preservative: | 0.01% (w/v) Sodium Azide |
Applications
Immunoblotting, ELISA, IHC
Recommended Dilution
This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,500 to 1:20,500 of the reconstitution concentration is suggested for this product. Optimal titers for applications should be determined by the researcher.
Immunogen
Human IgG whole molecule
Purification/Format
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
Background
Minimum Cross reactivity to Mouse Serum Proteins
Additional Information
Minimum Cross Reactivity to Mouse Serum Proteins
- Catalog Number
SA021-ABM - Supplier
ABM - Size
- Shipping
Blue Ice
Price
217,00 €
