pAAV-LK03 Vector

Product Description

AAV-LK03 Rep-Cap Plasmid

• Use this AAV Rep-Cap plasmid along with the AAV Helper Free System to produce recombinant human adeno associated virus (rAAV) virions without the need for a helper virus
• Ideal if you already have the AAV Helper Free System with a different AAV serotype
• Increase transduction efficiency of primary human hepatocytes

Adeno-associated virus (AAV) serotypes differ broadly in transduction efficacies and tissue tropisms. DNA family shuffling technology was used to create a complex library of hybrid capsids from ten different wild-type viruses. Stringent selection of AAV variants in vivo using FRG mice partially repopulated with primary human hepatocytes results in AAV-LK03. In comparison to AAV-DJ, which efficiently transduces most mouse and human cells in culture, AAV-LK03 preferentially transduces human cells. Importantly, AAV-LK03 transduces primary human hepatocytes 100-fold better than AAV8.