AAV-DJ Helper Free Promoterless Expression System

Product Description

• Complete Expression System contains packaging plasmids, expression vector and GFP control vector
• pHelper plasmid contains required E2A, E4, and VA RNA adenoviral genes; eliminates the need for a helper adenovirus
• AAV-DJ system provides a hybrid capsid created from 8 different AAV serotypes to provide substantially higher infectivity rates across a broad range of tissues
• Cloning capacity of expression vector = 3,9 kb

The AAV-DJ Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2¹.

1. Grimm, D. et al. (2008). J. Virol. 82: 5887-5911.

Vector Features

pAAV-DJ Vector
Vector Features:
6 ~ 1871: AAV-2 Rep
1888 ~ 4101: AAV-DJ Cap
5606 ~ 6466: Ampicillin Resistance

pHelper Vector
Vector Features:
1 ~ 5336: Adeno E2A
5337 ~ 8537: Adeno E4
8535~ 9280: Adeno VA
10182 ~ 11042: Ampicillin Resistance

GFP Control Vector
Vector Features:
1 ~ 130: Left ITR
139 ~ 801: CMV Promoter
809 ~ 1301: human β-globin intron
1324 ~ 2064: GFP
2123 ~ 2601: PolyA
2641 ~ 2781: Right ITR
3698 ~ 4558: Ampicillin Resistance