QuickTiter™ AAV Quantitation Kit

Product Description

• Ultra-fast procedure (<2 hours)
• Measures the viral nucleic acid content of unpurified AAV-2 or AAV-DJ supernatant or purified AAV of any serotype
• Limit of detection: 10^9 GC/mL from unpurified AAV supernatant or 5 x 10^10 GC/mL for purified AAV

Traditionally, adeno-associated virus (AAV) particles have been quantified by DNA dot blot or similar procedures. These methods can be time-consuming and suffer from a high degree of inter-assay variability.

Our QuickTiter™ AAV Quantitation Kit uses a proprietary technology to quantify the viral nucleic acid content of AAV preps. The kit can be used with unpurified supernatant of AAV-2 or AAV-DJ, or with purified AAV from any serotype.

Assay Principle

Fig.1. Assay Principle for the QuickTiter™ AAV Quantitation Kit.

Fig.2. Standard Curve Generated with the QuickTiter™ AAV Quantitation Kit. The QuickTiter™ AAV-2 DNA Standard was diluted as described in the Assay Protocol. Fluorescence measurement was performed on a SpectraMax Gemini XS Fluorometer (Molecular Devices) with a 485/538 nm filter set and 530 nm cutoff.