Rapid RCA Assay Kit

Product Description

• More accurate than traditional plaque-forming unit assays
• Faster results: 2.5 days vs. 10 days
• No agar overlay steps

Cell Biolabs Rapid RCA Assay Kit provides a quick and complete system to measure the titer of replication competent virus in your viral prep. In contrast to the 10-day infection of a classical plaque assay, the kit only requires 2-day infection. The kit provides sufficient reagents for up to 30 tests in 6-well culture plates.

Assay Principle

Performance Data

Fig.2. Staining Results from the Rapid RCA Assay. Serial 10-fold dilutions of Ad5 (2 x 10^12 viral particles/mL) were used to infect A549 cells in a 6-well plate for 48 hours. Anti-Hexon immunostaining was performed according to the assay protocol.