VitroGel Angiogenesis Assay HC Kit, TYPE 1

Tunable hydrogel system for 2D gel coating and 3D culture of angiogenesis tube formation, invasion, and animal injection

Product Description

VitroGel Angiogenesis Assay Kit is a revolutionary tool for researchers to study the effect of both hydrogel properties and culture medium on angiogenesis process. The kit can be used to study the angiogenesis tube formation and invasion on both 2D hydrogel coating method and 3D cell culture method. Cells cultured in this system can be harvested out with the VitroGel Cell Recovery Solution. The VitroGel system is also good for animal injection for in vivo study.

Traditional angiogenesis assay highly relies on natural extracellular matrix (natural ECM), which has non-adjustable hydrogel compositions and properties.  Therefore, our understanding of the angiogenesis process is limited by studying the molecular cues such as growth factors and inhibitors in culture medium only. There is a lack of knowledge on how the properties of hydrogel affect the angiogenesis process.

• Xeno-free functional hydrogel system supporting angiogenesis process
• Control and study the effect of the hydrogel mechanical properties on the angiogenesis process
• Multiple applications in one kit:  Tube formation, invasion, and animal injection
• Control the hydrogel properties:  Add your own growth factors and compare with positive control
• Harvest cells within 20 minutes at 37 °C without enzyme solution

Kit Content

• VitroGel AAK-HC, a tunable, xeno-free high concentration hydrogel
• AAK Dilution Solution, for adjusting the hydrogel concentration
• AAK Supplement 1, a hydrogel supplement without vascular endothelial growth factors (VEGFs) for cell attachment and growth
• AAK Supplement 2, a hydrogel supplement with VEGFs to promote tube formation and as a positive control
  
  

The VitroGel Angiogenesis Assay HC Kit is also available in other versions

VitroGel AKK-HC Kit Type 2 with AKK Supplement 1 only and VitroGel AKK-HC Kit Type 3 with AKK Supplement 2 only. In addition the VitroGel AKK-HC Hydrogel is available as a stand-alone product. Please contact us if you are interested.

2D Hydrogel Coating for Tube Formation and Invasion Workflow

3D cell Culture Workflow