Minute Detergent- and EDTA-free Total Protein Extraction Kit for Mammalian and Insect Cells and Vertebrate and Invertrebrate Tissue (Denatured or Native Proteins obtained)

Product Description

Minute™ Detergent-Free Protein Extraction Kit

(For Animal Cultured Cells and Tissues)

Invent Biotechnologies' Minute™ detergent-free total protein extraction kit is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract detergent-free total proteins from cultured cells (insect /mammalian/other cultured cells) and animal tissues (invertebrate and vertebrate). The protein extraction buffers do not contain any detergent and EDTA. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 20 ìl and as large as 500 ìl. This unique feature is very useful in situations where available starting material is a limiting factor. Detergent-free total proteins can be extracted from cultured cells/tissues in less than 5 min with high yield (1-5 mg/ml).

Figure 1. Comparison of protein profiles of different animal tissues extracted by denaturing lysis buffer and detergent-free extraction buffer. Lane 1, Drosophila cultured cells (SL2); Lane 2, Drosophila adult flies; Lane 3, gold fish muscle; Lane 4, mouse liver. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.