EpiMag Viral RNA Isolation Kit Add to Cart

Cat#: P-9108-050-EP
Quantity: 50 rxns
Price: 164 €
Supplier: Epigentek
Shipping: RT
Sample Type: Cell-free liquid specimens, specifically from saliva and nasal or nasopharyngeal swabs
User Manual  

Magnetic bead-based extraction of total viral RNA from saliva and nasopharyngeal swabs

• Fast procedure delivers high-quality total RNA in 25 minutes
• Allows small size (>30 bases) and large size (<200 kb) to be isolated
• Convenient handling without the need for centrifugation
• Ready-to-use RNA for high performance in downstream application
• Consistent RNA yield from a small amount of starting material

The EpiMag™ Viral RNA Isolation Kit is a complete set of optimized buffers and reagents suitable for quick preparation of viral RNA from cell-free liquid specimens, specifically from saliva and nasal or nasopharyngeal swabs. The specialized buffering system allows RNA to bind to the magnetic beads while contaminants and impurities are efficiently washed away, and pure RNA is eluted. The RNA purified with the kit is suitable for a variety of routine applications, specifically for RT-PCR.

Principle & Procedure
The kit contains all the reagents required for successfully performing RNA isolation directly from cell-free samples. After lysis, binding, and wash, RNA is easily recovered in quantities of up to 5 µg using specially designed magnetic beads. Total RNA is then ready to be used for a variety of downstream applications.

Starting Materials
The amount of starting materials can be up to 400 µl of liquid volume, with the best volume of 200 µl. A total of 50 standard extractions (using 200 µl of the sample) can be performed with this kit.


Image
Fig.1.
Total RNA isolated from nasal swab samples with EpiMag™ Viral RNA Isolation Kit and was used for RT-PCR analysis. A: Nasopharyngeal swab sample was spiked with SARS-CoV-2-N positive control; B: Nasopharyngeal swab sample was not spiked with SARS-CoV-2-N positive control. PCR was processed with use of primers and probes against SARS-CoV-2 N1/N2 and human RNase P gene (internal control).


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