EpiQuik Viral RNA Isolation Fast Kit

Product Description

• Fast procedure delivers high-quality total RNA within 10 minutes
• Consistent RNA yields from small amounts of starting material
• High performance in many downstream applications, especially RT-PCR

The EpiQuik™ Viral RNA Isolation Fast Kit is a complete set of optimized buffers and reagents suitable for quick preparation of viral RNA from cell-free liquid specimens, specifically from saliva and nasal or nasopharyngeal swabs. The specialized buffering system allows RNA to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and pure RNA is eluted. The RNA purified with the kit can be used for a variety of routine applications, specifically for RT-PCR.

Principle & Procedure

The kit contains all the reagents required for successfully performing RNA isolation directly from cell-free samples. After lysis, binding, and wash, RNA is easily recovered in quantities of up to 5 µg using specially designed columns. Total RNA is then ready to be used for a variety of downstream applications.

Starting Material

The amount of starting materials can be up to 400 µl of liquid volume, the optimal volume being 200 µl. A total of 50 standard extractions (using 200 µl of sample) can be performed with this kit.

Binding Capacity and Yield

The column binding capacity can be up to 5 µg. RNA spiking tests show that the yield is >95%. However, the yield from different samples may vary depending on the sample type.

Fig.1. Quantification analysis of the isolated RNA. 200 nt RNA fragment at different concentrations was spiked into normal saline and then isolated with the EpiQuik™ Viral RNA Isolation Fast Kit. Isolated RNA was then fluorescently quantified using a fluorescent method for RNA/ssDNA measurement.

Fig.2. Total RNA isolated from nasal swab samples with EpiQuik™ Viral RNA Isolation Fast Kit and was used for RT-PCR analysis. A: Nasopharyngeal swab samples were spiked with SARS-CoV-2-N positive control; B: Nasopharyngeal swab samples were not spiked with SARS-CoV-2-N positive control. PCR was processed with use of primers and probes against SARS-CoV-2 N1/N2 and human RNase P gene (internal control).