Air-Dryable™ Direct DNA qPCR Plant

Designed for creating ambient temperature stable assays from plant material without extraction

Product Description

Air-Dryable™ Direct DNA qPCR Plant is a glycerol-free, inhibitor-tolerant master mix that contains optimized excipients compatible with air and oven drying. It has been designed for the development of qPCR assays for direct detection of plant DNA from crude lysate without extraction. Can also be used wet and/or with purified nucleic acid.

Air-Dryable™ Direct qPCR Plant is the first commercially available mix that combines the benefits of inhibitor-tolerance with air-drying, making it ideal for manufacturing room-temperature stable, highly sensitive and cost-effective plant assays. For more details please see the user manual above and the Air-drying User Guideline.

A single leaf punch can then be lysed in hot SDS lysis buffer, alkaline buffer or water and added directly onto the dried assay for the detection with very high sensitivity and reproducibility.

Product Highlights

• Suitable for direct DNA detection from plant lysates
• High performance of both wet and dried formats

Compatible with simple, direct workflows

Fig.1. A single tomato leaf punch was added to 20 μL water and heated to 95 °C for 5 minutes. The total lysate was then added to Air-Dryable™ Direct DNA qPCR Plant mix that had been air-dried with ARF2 primers and probe. The traces from 8 individual leaf punches (brown) are shown overlaid with tomato gDNA standards (blue) for reference to show that approximately 84 genome equivalents per leaf punch were detected. The results illustrate the speed and reproducibility of the Air-Dryable™ Direct DNA qPCR Plant even with non-complex assays.