Liquid Biopsy Direct qPCR & RT-qPCR Master Mixes
Oncology testing of crude blood, saliva, urine, and stool samples
Liquid biopsy collects samples such as blood, urine, saliva and stool and is significantly less intrusive than surgical biopsy. It provides access to cell-free DNA (cfDNA), enabling early detection of cancer cells (before a tumour develops) and together with RNA detects non-shedding cancers. Liquid biopsies provide an easier and safer mode to monitoring disease recurrence, therapeutic selection and effectiveness.
- qPCR mixes designed for direct amplification from crude samples eliminate DNA/RNA extraction
- Innovative chemistries offer ultra-sensitive detection of cfDNA, ctDNA and ctRNA
Lyo-Ready & Air-Dryable qPCR/RT-qPCR Master Mixes
Molecular diagnostic tests are progressively moving towards lyophilized and air-dried formats. Lyo-Ready and Air-Dryable qPCR master mixes are 4x concentrated, glycerol-free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient-temperature stable assays, or they can be used as a wet mix. They are highly inhibitor-tolerant and ultra-sensitive making them ideal for point-of-care or machine-automated multiplexing assays. As ready-to-use mixes, they require no further optimization besides the addition of primers and probes.
Inhibitor-Tolerant qPCR/RT-qPCR Master Mixes
Use this Universal inhibitor-tolerant qPCR/RT-qPCR Master Mixes, for direct amplification from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood, sputum, saliva and stool.
PCR inhibitors are common in clinical and environmental samples—and even with standard processing and extraction, they can still undermine assay performance. Traditional workflows are not fully efficient, often reducing the amount of target nucleic acid available for detection.
Inhibitor-Tolerant qPCR/RT-qPCR Master Mixes are also available in a Lyo-Ready Inhibitor-Tolerant format, which are glycerol-free and contain lyophilization compatible excipients.
Direct DNA Detection from Plant Lysates
The Air-Dryable Direct DNA qPCR Plant master mix is designed for the development of ambient temperature qPCR assays for direct detection of plant DNA from crude lysates without extraction.