cfPure Cell Free DNA Extraction Kit, 100 ml
| Specifications | |
|---|---|
| Product Category: | Circulating DNA Purification |
| Sample Type: | Plasma |
Product Description
BioChain's cfPure™ Cell Free DNA Purification Kits are designed for rapid and efficient isolation of circulating cell free DNA (cfDNA).
- Versatile: Extraction from <1mL to >10mL serum or plasma – in single tubes or 24- to 96-well plates
- Automation Friendly: On both the KingFisher™ and liquid handling instruments, with extractions taking approximately 50 minutes
- Time Efficient: Manual extraction protocol takes less than 75 minutes
- Elution volumes: Range from 15 µL to 50 µL
The cfPure® Cell Free DNA Extraction system utilizes silica-coated paramagnetic particles to purify cell free DNA from less than 1 mL to greater than 10 mL of serum or plasma. The buffers were developed to ensure efficient recovery of 100 bp – 500 bp DNA fragments in order to maximize recovery of cell free DNA. The recovered DNA is suitable for a wide range of down-stream applications, such as bisulfite sequencing, NGS, and qPCR.
In general, this cfPure kit performs best with higher yields and complete cfDNA capture when double-spun plasma that is free of contaminating white blood cells is used. However, if the plasma
was not double-spun immediately upon collection or there is other gDNA contamination in the plasma, then we strongly recommend using the cfPure V2 kit instead. V2 will minimize the gDNA contamination in the final elution, although it should be noted that some of the cfDNA may also be lost.
Rapid and Efficient Magnetic Bead-Based Protocol
Figure 1. The cfPure® Cell Free DNA Extraction Kit efficiently recover important small fragments (100-500bp).
The cfPure® protocol is rapid and easy, allowing users to process several samples in an hour or less and making it ideal for biomarker screening. BioChain has developed its own silica-coated magnetic bead technology, which is designed to efficiently recover low molecular weight cell free DNA during isolation. In addition to being easy to perform, and easy to automate, you can use less magnetic bead solution if you are purifying small amounts of plasma – saving you money. The key to successful analysis of cfDNA is to ensure efficient purification of smaller DNA fragments (100-500 bp). As Figure 1 shows, the cfPure® Cell Free DNA Extraction Kit does efficiently recover these important small fragments.
Product Citations
- Mattox AK et al. (2023) The origin of highly elevated cell-free DNA in healthy individuals and patients with pancreatic, colorectal, lung, or ovarian cancer. Cancer Discov. 13(10):2166–2179.
