OneScript Plus Reverse Transcriptase

Product Description

OneScript^® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates.

OneScript^® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

• High Processivity: Generate long full-length cDNAs up to 15 kb
• High Sensitivity: Amplify rare or low copy transcripts
• High thermostability at 50-55°C/122-131°F
• 10-15 minutes reaction time

Applications

• Synthesizing cDNA from a ssRNA
• DNA primer extension
• Sequencing dsDNA
• Constructing cDNA library
• Constructing libraries for serial analysis for gene expression (SAGE)
• Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
• Producing template for use in RT-PCR or real-time RT-PCR
• Labelling 3'-end of duplex DNA via end-filling reactions
• Generating probes for hybridization

OneScript® Plus Reverse Transcriptase elongation ability

Human cDNA synthesized by OneScript® Plus Reverse Transcriptase, followed by PCR and 1% agarose gel electrophoresis

A complete High Efficiency cDNA Synthesis kit with OneScript Plus RT, incl. recombinant RNasin™ Ribonuclease Inhibitor, oligo(dT) and random primers, dNTPs, and RNAse free water, is also available