OneScript Plus cDNA Synthesis Kit

Product Description

OneScript^® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript^® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

• Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
• Robust cDNA synthesis from any RNA template
• High reproducibility and excellent yield

Choose your priming methods with abm’s cDNA Synthesis Kit. abm’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality DNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.

Applications

• Synthesizing cDNA from a ssRNA
• DNA primer extension
• Sequencing dsDNA
• Constructing cDNA library
• Producing template for use in RT-PCR or real-time RT-PCR
• Labelling 3'-end of duplex DNA via end-filling reactions
• Generating probes for hybridization

OneScript® Plus Reverse Transcriptase elongation ability

Human cDNA synthesized by OneScript® Plus Reverse Transcriptase, followed by PCR and 1% agarose gel electrophoresis

The OneScript Plus High Efficiency cDNA Synthesis Kit is a complete system for the efficient synthesis of long first strand cDNA from RNA templates with secondary structures and high GC contents. The recombinant RNasin™ Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation. The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5’-end regions of mRNA. Gene-specific primers may also be used with the kit. Obtained first strand cDNA can be directly used as a template in PCR and real time PCR.

OneScript Plus High Efficiency Reverse Transcriptase is also available as a separate product.