Exo-Check Exosome Antibody Array (Neuro) Standard

Product Description

The Exo-Check Exosome Antibody Array (Neuro) enables a quick and efficient identification of exosomes carrying neuronal markers of both exosome-specific and neuron-specific markers in a single experiment.

• Use as little as 10 µg of input protein for profiling general EV and neuronal-associated markers
• Provided in a simple, easy-to-use membrane-based dot-blot array format
• Profile up to 11 targets and 3 controls without the hassle of western blotting
• Suitable for EVs isolated using column-based/SEC methods, ultracentrifugation, and precipitation, as well as immuno-affinity captured EVs
• Targets are carefully chosen from the literature and have been shown to be associated with EVs
  
  

Supporting data

Detection of Serum Exosomes

Fig.1. Sample Exo-Check Antibody Array (Neuro) data showing exosome detection and confirmation of the presence of neuronal markers. (A) EVs were isolated from 250 µl of pooled human serum using SBI’s ExoQuick Reagent, and 10 µg of EV-associated protein were loaded onto a standard Exo-Check Neuro Array. 

Sensitivity

Fig.2. Detection results with different loadings. The Exo-Check Neuro Array is sensitive, providing robust and reproducible signal from as a little as 10 µg of input sample. EVs were isolated from 250 µl of human serum using ExoQuick Reagent ,and either 10 µg, 20 µg, or 50 µg of EV-associated protein were loaded onto a standard array.