Nuclear/Cytosolic Fractionation Kit
| Specifications | |
|---|---|
| Product Category: | Cell Fractionation |
| Sample Type: | Mammalian Cells |
Product Description
- Simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells
- Extraction in less than 2 hours
- Each prep can accommodate up to 5 x 106cells
Cell Biolabs’ Nuclear/Cytosolic Fractionation Kit provides a simple and fast tool to isolate nuclear extract from the cytoplasmic fraction of mammalian cells. The procedure has been optimized to provide extraction, with high protein recovery and low cross-contamination, in less than 2 hours. The extracted protein fractions are functional and suitable for downstream assays such as DNA footprinting, RNA splicing, gel shift assays (EMSA), reporter assays, enzyme activity assays, and Western blotting.
Supporting Data
Fig.1. HEK293 Cell Fractionation. Cytosolic and nuclear protein extracts were isolated from Human Embryonic Kidney 293 cells according to the Assay Protocol. Whole cell (W), cytosol (C), and nuclear (N) fractions were immunoblotted with Anti-α-Tubulin (cytosol-specific protein,left) or Anti-Lamin A/C (nuclear-specific protein, right) at 1 µg/mL.
Fig.2. HEK293 Trypan Blue Staining. Human Embryonic Kidney 293 cells were stained with Trypan Blue at various steps during the fractionation protocol, demonstrating complete lysis and high neuclei recovery.
