ORF1 Polyclonal Antibody, Biotin Conjugated
| Specifications | |
|---|---|
| Type: | Primary Antibody |
| Clonality: | Polyclonal |
| Host: | Rabbit |
| Reactivity: | Hepatitis E virus genotype 1 |
| Isotype: | IgG |
| Label: | Biotin |
| Additional Information | |
|---|---|
| Synonyms: | Methyltransferase , Putative papain-like cysteine protease, NTPase/helicase, RNA-directed RNA polymerase, RdRp, ORF1 |
| UniProt ID: | P33424 |
Applications
ELISA
Recommended Dilution
Recommended dilution: ELISA 1:500-1:1000
Immunogen
Recombinant Hepatitis E virus genotype 1 Non-structural polyprotein pORF1 protein (60-240AA)
Purification/Format
Protein G purified
Background
Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction.
Price
591,00 €
