Saliva/Swab RNA Purification Kit, column format Add to Cart

Cat#: 69100-NB
Quantity: 50 rxns
Price: 343 €
Supplier: Norgen
Shipping: RT
User Manual  

• For High quality total RNA, including viral RNA,
• For fresh and preserved saliva and swab samples
• Fast and easy processing using rapid spin-column format
• Isolate total RNA, from large rRNA down to microRNA (miRNA)
• No phenol or chloroform extractions
• Even suitable for a few cells/viral copies without the need for carrier RNA
• Purified RNA for many downstream applications, incl. Small RNA Sequencing
CE-IVD marked version available

Norgen’s Saliva/Swab RNA Purification Kit provides a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes. Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The chemistry employed in the kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is ideal for in vitro diagnostic use for medical purposes.


Average RNA Yield of Samples Isolated by Norgens Saliva/Swab RNA Purification Kit

Image
Figure 1. The average yield of RNA isolated by Norgens Saliva/Swab RNA Purification Kit is ≥ 1 µg. Duplicate preserved (P) and non-preserved (NP) swab (nasal and oral) and saliva samples were collected from different donors. Preserved swab samples (nasal or oral) were collected into Norgens Total Nucleic Acid Preservative Tubes (Cat #69200) and preserved saliva samples were collected using Norgens Saliva RNA Collection and Preservation Devices (Cat #RU53800). Non-preserved samples were processed immediately without mixing with any preservative. All collected samples were processed for RNA isolation using Norgens Saliva/Swab RNA Purification Kit, using an input of 0.25 mL from preserved samples and the non-preserved saliva or the entire non-preserved swab for the non-preserved swabs. NanoDrop was used to estimate the concentration and yield of the isolated RNA.

Related Links

Tutorial Saliva RNA Extraction


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