RNA/DNA/Protein Purification Plus Kit - 96 Well Format

Product Description

• Isolate high quality and high yield total RNA and Genomic DNA & Proteins
• The only 96-well kit available that also purifies proteins
• Sequentially isolate nucleic acids and proteins from a single lysate – no need to split the lysate
• Isolate total RNA, from large rRNA down to microRNA (miRNA)
• No phenol or chloroform extractions
• Fast and easy processing using either a vacuum manifold or centrifugation
• Isolate from small samples - Sequential isolation of RNA, DNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures.

Norgen’s RNA/DNA/Protein Purification 96-Well Kit provides a rapid method for the high throughput isolation and purification of total RNA, genomic DNA and proteins simultaneously from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines.

Norgen’s RNA/DNA/Protein Purification 96-Well Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Performance Data

Figure 1. High Throughput Isolation of High Quality RNA with Complete Size Range without the Use of Phenol. Norgen's RNA/DNAProtein Purification 96-Well Kit allows for the consistent isolation of high quality RNA, with complete size range from the very large RNA down to small RNA without the use of phenol. HeLa RNA and Hamster Liver RNA was isolated using Norgen’s RNA/DNA/Protein Purification 96-Well Kit in replicates. (A) The isolated RNA was resolved on a 1.2% formaldehyde agarose gel. All replicates showed both high yield and high quality. In addition, all replicates showed effective recovery of all sizes of RNA including the small RNA (arrow). (B) The isolated RNA was resolved on an Agilen RNA Nano 6000 Chip. All RNA achieved high RNA Integrity Number (RIN). The RNA isolated by Norgen’s RNA/DNA/Protein Purification 96-Well Kit showed good recovery of both small RNA (miR-21, (C)) and large RNA (beta-Actin, (D)).

Figure 2. Recovery of Intact, High Quality Genomic DNA from HeLa cells and Hamster Liver. Panel A is a 1% agarose gel showing the gDNA isolated from the same HeLa cell or hamster liver samples using Norgen’s RNA/DNA/Protein Purification 96-Well Kit with Norgen’s HighRanger 1 kb DNA Ladder and the sample lanes contain 10 µL of each of the 100 µL elutions. The gel showed high quality, and intact genomic DNA. Panel B is the result of qPCR amplification of 25 ng of eluted genomic DNA using 5S rRNA-specific primers. Genomic DNA isolated using Norgen’s RNA/DNA/Protein Purification 96-Well Kit is of high quality and is compatible to sensitive downstream applications.

Figure 3. Consistent Isolation and Purification of Total Protein from the same sample used for RNA and DNA Isolation. Norgen’s RNA/DNA/Protein Purification 96-Well Kit allows concurrent isolation of RNA, DNA and protein from the same sample without splitting. The flowthrough samples from the RNA/DNA isolation in Figure 1 and Figure 2 were further purified using Norgen’s RNA/DNA/Protein Purification 96-Well Kit. Ten microliters of the 100 µL protein elutions were loaded on a 10% SDS-PAGE gel. The proteins are consistent in yield, intact and of the highest quality, and can be used in a number of different downstream applications.