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Total RNA Purification PLUS Kit (Chromatography Columns and additional gDNA removal columns for rapid total removal of any residual genomic DNA) for mammalian cells and tissue, blood, plasma / serum, bacteria, yeast, fungi, plants, and more Add to Cart

Cat#: 48300-NB
Quantity: 50 preps
Price: 355 €
Supplier: Norgen
Shipping: RT
User Manual  

• Isolate true total RNA including siRNA and microRNA
• Rapidly remove contaminating genomic DNA without the use of enzymes
• Isolated RNA is suitable for RNA Sequencing projects
• No phenol or chloroform extractions
• Purify high-quality RNA in 20 minutes
• Extract RNA from as little as a single cell
• Isolate from a wide variety of specimens

The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), without the use of phenol or chloroform. Genomic DNA is removed from the sample using a Genomic DNA Removal column, and the RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. Norgen´s kit is the only kit on the market that isolates true total RNA, as other kits must use phenol to recover all sizes of RNA.

Total RNA can be isolated from a broad range of sample sources with this kit including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, plants and viruses. Norgen´s Total RNA Purification Plus Kit works very well with small sample inputs and does not require carrier RNA for the extraction. Total RNA can be isolated from as little as a single cell using this kit.

Procedure:
Image

Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.


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