Animal Tissue RNA Purification Kit for mammalian tissue

Product Description

• Fast and easy processing - The Rapid spin-column format allows for the processing of 10 samples in 50 minutes
• Isolate a diversity of RNA species - All RNA species, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA) can be isolated
• No phenol or chloroform extractions
• Purified RNA is of the highest quality and integrity
• Isolation and purification of total RNA from all types of animal tissue samples including difficult to extract fibrous tissues such as heart and muscle

Norgen’s Animal Tissue RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from all types of animal tissue samples, including fiber-rich tissues such as muscle and heart. The kit can isolate RNA from fresh or flash-frozen tissues, as well as tissues stored in RNA preservation reagents such as RNAlater®. Norgen’s Animal Tissue RNA Purification Kit is provided with Proteinase K, which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins and connective tissues. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), without the use of inhibitory phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Briefly, the tissue of interest is first lysed using the Lysis Solution, followed by treatment with the provided Proteinase K. Ethanol is then added, and the RNA is bound to Norgen's column (BIND). Under these conditions only the RNA will bind to Norgen's resin while most of the contaminating cellular proteins are removed in the flowthrough or retained on top of the resin. At this point an optional on-column DNase digestion can be performed using the provided DNase to remove any traces of DNA which may co-purify with the RNA. The bound RNA is then washed to remove any remaining impurities (WASH). Lastly, the purified total RNA is eluted into the provided Elution Buffer or water (ELUTE).

Workflow