CUTANA DNA Purification Beads

Exceptional recovery with minimal bias or carry-over contamination

Product Description

CUTANA™ DNA Purification Beads harness Solid-Phase Reversible Immobilization (SPRI*) technology to deliver fast, high-yield DNA cleanup and precise size selection for NGS library preparation and other genomic workflows.

• Trusted method – SPRI chemistry researchers rely on for DNA cleanup
• NGS validated – proven performance in genomic workflows including CUT&RUN, CUT&Tag, and library preparation
• High recovery & purity – consistently recovers DNA across a wide range of fragments and input amounts
• Automation-compatible – suitable for manual or automated protocols using magnetic separation
• Drop-in AMPure XP* alternative – identical ratios for seamless replacement in SPRI-based cleanups
• Room-temperature stable – ready to use; no warmup period required

Optimized PEG and salt concentrations drive efficient, reproducible binding of DNA to paramagnetic beads, ensuring exceptional recovery with minimal bias or carry-over contamination. The formulation matches the bead-to-sample ratio of popular alternatives such as AMPure XP* and SPRIselect*, ensuring CUTANA™ DNA Purification Beads can serve as a drop-in replacement into manual or automated cleanup workflows without any protocol re-optimization.

*SPRI, SPRIselect, and AMPureXP are registered trademarks of Beckman Coulter and nominative references to them are for descriptive purposes only. CUTANA™ products are not manufactured by or affiliated with Beckman Coulter.

Validated Applications

• CUT&RUN
• CUT&Tag
• NGS Library Preparation & adapter dimer cleanup

Performance Data

Figure 1: DNA Fragment Recovery Cleanup. Representative gel images show recovery of various DNA fragment sizes generated using CUTANA™ DNA Purification beads compared to Competitor A. CUTANA™ DNA Purification Beads were subjected to a magnetic bead-based DNA cleanup protocol using a GeneRuler 50 bp DNA Ladder (ThermoFisher Scientific SM0371) tested at a range of bead-to-sample ratios (0.8 – 1.8X). The purified DNA was run on an Agilent TapeStation.

Figure 2: Magnetic Bead-based Cleanup of Adapter Dimers from Genomic Sequencing Libraries. Adapter dimers (~125 bp) result from self-ligation of sequencing adapters that may be preferentially amplified in PCR. They are particularly problematic at low cell inputs, where genomic DNA templates are scarce. Adapter dimers occupy valuable sequencing bandwidth and should be removed whenever possible. Agilent TapeStation revealed adapter dimers present in CUT&RUN libraries generated using 5k and 17k K562 cells (A). CUTANA™ DNA Purification Beads were used for DNA size selection (see Application Notes) on the pooled sequencing library, resulting in high-quality ready-for-sequencing libraries (B).