CUTANA™ Nuclei Extraction Buffer

Product Description

CUTANA™ Nuclei Extraction Buffer is the essential reagent for harvesting nuclei from cultured cells and tissues for use in CUT&RUN and CUT&Tag assays.

This buffer is expected to be broadly compatible with eukaryotic cells and tissues. Below are examples of various cells and tissue types that have undergone successful nuclei isolation using this buffer:

The reagent is prepared by supplementing Pre-Nuclei Extraction Buffer with protease inhibitor and spermidine fresh on the day of use. Utilize this buffer in EpiCypher’s CUTANA™ Nuclei Extraction Protocol for CUT&RUN to obtain the highest quality nuclei for your genomic mapping assay.

Supporting Data

Figure 1: Nuclei Extraction. Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.

Recommended Accessory Products

CUTANA™ ChIC/CUT&RUN Kit
CUTANA™ pAG-MNase for ChIC/CUT&RUN
CUTANA™ pAG-Tn5 for CUT&Tag

NOTE: CUTANA™ CUT&Tag Kits (14-1102-EPC/14-1103-EPC) already contain Nuclei Extraction Buffer.