Anti-monkey/Pig/Dog Involucrin Antibody

IHC (Prediluted format)

Purified Involucrin from human keratinocytes was used as the immunogen for this antibody.

This antibody recognizes a protein of 66-17kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this antibody reacts with a 12kDa protein. It stains the involucrin in a variety of sizes: 17kDa in MCF-7 cells, a doublet of ~115 and 15kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 15kDa in pig. Its epitope maps between amino acids 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.