ProteoSpin Serum Depletion Kit

For Rapid Depletion of Abundant Proteins from Serum Samples

Product Description

• Rapid and efficient removal of abundant proteins from serum and plasma samples
• Process 10 samples in 30 minutes
• Allows for visualization of low abundance proteins
• Convenient and affordable spin column protocol
• Generic protocol allows for depletion from human and other animal samples
• Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix

This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples.  Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover.  The kit reduces sample complexity to allow for convenient investigation of less abundant proteins.  In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins.  The kit is 'generic' in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material - human or various other animals - making it more versatile, flexible and affordable than other kits.   Resulting proteins are ready for a range of

Applications

Resulting proteins are ready for a range of applications:

• 2D gel electrophoresis
• SDS-PAGE
• Mass  Spectrometry
• Protein microarrays
• DIGE (Difference Gel Electrophoresis)
• and more

Kit Performance

Figure 1. Depletion of Abundant Proteins. Abundant proteins are depleted from human serum to a large degree using the ProteoSpin™ Abundant Serum Protein Depletion Kit. Lane A represents the input human serum proteins, while Lanes C and D represent the elution after the abundant proteins were depleted with the kit. Lane B represents the binding flowthrough, and contains the proteins that have been depleted from the serum sample.