BRM/SMARCA2 CUTANA CUT&RUN Antibody

CUTANA Compatible Antibodies meet the criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping.

Product Description

BRM antibody produces CUT&RUN peaks above background localized to gene transcription start sites (Figure 1), cconsistent with its known role as the ATP-dependent helicase subunit of the SWI/SNF chromatin remodeler complex (1).

Figure 1: BRM enrichment at annotated transcription start sites (TSSs) in CUT&RUN. CUT&RUN was performed using 500,000 K562 cells with BRM (0.1 µg) and control antibodies (0.5 µg; IgG, EpiCypher 13-0042; H3K4me3, EpiCypher 13-0041). Sequencing reads were aligned to TSSs (+/- 2 kbp) of 18,793 genes. Signal (red) over background (blue) is ranked by intensity (top to bottom). All rows aligned to BRM antibody with moderate fixation (0.1% formaldehyde, 1 min), which improved signal vs. native conditions.

Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein.

References

1. Raab et al (2017) Epigenetics Chromatin 10:62.