CUTANA™ Anti-GST Tag Antibody

Product Description

Unlock the full potential of Reader CUT&RUN with our high-affinity CUTANA™ Anti-GST Tag Antibody, specifically developed to support chromatin profiling applications wherein a GST-tagged reader protein replaces the traditional primary antibody in directing the selective cleavage and release of target chromatin fragments by pAG-MNase [1]. For example, this antibody is used in CUTANA™ meCUT&RUN (Cat# 14-1060-24-EPC), a modified CUT&RUN workflow in which a GSTtagged MeCP2 methyl binding domain is used to enrich regions of chromatin with symmetrically methylated CpGs, enabling streamlined, high-resolution mapping of DNA methylation.

Engineered for superior performance in low-background, high-resolution CUT&RUN assays, this antibody offers exceptional sensitivity and specificity for detecting GST-fusion proteins bound to chromatin. Whether studying histone modifications, DNA methylation, or other epigenetic readers, this antibody is the ideal tool for enabling precise, reproducible mapping using GST-tagged fusion constructs.

Fig.1: meCUT&RUN DNA fragment size distribution analysis. meCUT&RUN was performed as described in the datasheet. Library DNA was analyzed by Agilent TapeStation®. This analysis confirmed that mononucleosomes were predominantly enriched in meCUT&RUN (~300 bp peaks represent 150 bp nucleosomes + sequencing adapters).