ProteoSpin Micro Inclusion Body Isolation Kit Add to Cart

Cat#: 10300-NB
Quantity: 20 rxns
Price: 360 €
Supplier: Norgen
Shipping: RT
User Manual  

Obtain and solubilize inclusion bodies and purify the proteins all with 1 kit

The ProteoSpin™ Inclusion Body Isolation Micro Kit facilitates the screening of E. coli clones that express recombinant proteins in inclusion bodies. The kit includes reagents specially formulated to achieve rapid and high-quality purification of inclusion bodies using three processes:

Lysis of bacterial cells to release inclusion bodies in solid form
Solubilization of purified inclusion bodies
Purification of the recombinant protein using spin column chromatography

Downstream applications include SDS-PAGE analysis, refolding experiments, mass spectrometry, further purification and scale up.

The ProteoSpin™ Inclusion Body Isolation Micro Kit employs spin-column chromatography using Norgen’s patented protein resin as an ion exchanger. Each spin column can isolate up to 50 ug of either acidic or basic protein from 1.5 mL of bacterial culture.
Capacity per spin tube is approximately 2-50 ug and may vary depending on protein sample.
The protocol was designed for use with 1.5 mL of bacterial cultures harvested at the end of the protein induction period.

Image

Time course study for expression of a 40 kDa protein. Expression was induced with 0.4 mM IPTG and incubated at 37 °C for three hours. Aliquots were removed at indicated intervals and purified. Proteins made visible by Coomassie Blue staining.

ProteoSpin™ Inclusion Body Isolation Micro Kit Contents

Each ProteoSpin™ Inclusion Body Isolation Micro Kit provides the following essential components for use with the provided protocols:

Cell Lysis reagent (also available seperately, see link below)
Inclusion Body solubilization reagent (also available seperately, see link below)
Column activation and wash buffer for acidic proteins
pH binding buffer for acidic proteins
Column activation and wash buffer for basic proteins
pH binding buffer for basic proteins
Elution buffer
Neutralizer
ProteoSpin™ micro spin columns
Collection tubes (2 mL capacity)
Final collection tubes (1.7 mL capacity)
Syringes (1 mL capacity)
Needles (20 gauge)
ProteoSpin™ Inclusion Body Isolation Micro Kit application manual
Protocol reference card for both acidic and basic procedures


Customer-supplied Reagents and Equipment:

Benchtop microcentrifuge
Micropipetters
Sterile, deionized water or Milli-Q water


ProteoSpin Inclusion Body Isolation Kit Benefits

* Three kits in one for acidic and basic proteins -
The kit provides essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography. The kit includes solutions and protocols for use with either acidic or basic proteins.

* Gentle disruption procedure provides high purity final product - Cell lysis is accomplished through non-ionic detergent chemical disruption. The use of a needle and syringe reduces viscosity and facilitates purification, producing protein often >95% pure.
Proteins are then ready for SDS-PAGE, 2D gels, mass spectrometry analysis or other procedures.

* Convenient process volume - Analyze 1.5 mL of microbial cell culture yielding 2–50 ug of protein.
* Long shelf life - The kit uses an enzyme-free process with stable solutions for longer shelf life.
* Fast process time - Process up to 12 samples in only 60 minutes.

Applications

Mass Spectrometry
SDS-PAGE
Refolding Experiments

Related Links

Macro Kit for 100 ml culture also available

Downloads - Will open in new browser window

Product Information
Application: Screening for E. coli Inclusion Bodies
Application: Time Course of Protein Expression


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