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Cell-Free Circulating RNA and DNA

Cell-Free Circulating RNA and DNA Purification and Preservation Kits

Purification

Cell-Free Circulating (CFC) DNA and RNA Purification Kits developed by Norgen provide an efficient method for the purification of fragmented free-circulating DNA, RNA, exosomal RNA or total nucleic acids from variable volumes of plasma/serum or cell-free urine samples. The kits are able to isolate all sizes of circulating DNA or RNA irrespective of size or GC content, without bias. These kits provide an advantage over other available kits in that they do not require extension tubes for the purification of cfc DNA or RNA from large sample volumes. The purified cfc DNA or RNA are eluted in an elution solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern blot analysis, reverse transcription PCR, Northern blotting, RNase protection, primer extension and expression array assays.

Preservation

The Norgen cf-DNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cell-free circulating DNA/circulating tumor DNA in human whole blood samples during storage and shipping. The cell-free circulating DNA/circulating tumor DNA levels are stable for up to 30 days at room temperature or up to 8 days at 37°C. Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA Preservative Tubes and purified using Norgen's different Plasma/Serum DNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA for any downstream application assays

Quantification

Since measuring the cfc-RNA and cfc-DNA concentrations from cell-free bodily fluids using common quantification methods is very difficult and challenging due to their low amounts, our partner Norgen has developed two PCR-based quantification kits for the accurate and reliable quantification of cfc-DNA and cfc-RNA from samples that normally contain very low nucleic acid amounts. These kits offer a PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range. The unknown DNA or RNA is accurately quantified by using a standard curve constructed from the provided DNA/RNA Standards.

Characteristics

  • Isolate all sizes of cfc-DNA, cfc-RNA, and exosomal RNA, including microRNA
  • Various sample types: Plasma/Serum, Urine, CSF and other bodily fluids
  • Concentrate circulating DNA into a flexible elution volume ranging from (10 µl - 100 µl)
  • No phenol extractions nor carrier RNA
  • Inhibitor-free cfc-DNA and cfc-RNA
  • Fixative-free preservative, no cross-linking of DNA
  • Preserve cell-free DNA/circulating tumor DNA for 30 days at ambient temperature
  • No plasma volume loss after shipping/transportation

Cell-free DNA Preservation

Blood cf-DNA/cf-RNA Preservative Tubes 63950-NB 50 tubes 497 €   DETAILS

DNA Purification Kits

Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Micro Kit 55500-NB 50 preps 246 €   DETAILS
Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Mini Kit 55100-NB 50 preps 246 €   DETAILS
Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Midi Kit 55600-NB 20 preps 437 €   DETAILS
Plasma/Serum Cell-Free Circulating DNA (cfc DNA, ccf DNA) Purification Maxi Kit 55800-NB 10 preps 332 €   DETAILS
Urine Cell-Free Circulating DNA Purification Mini Kit 56600-NB 50 preps 276 €   DETAILS
Urine Cell-Free Circulating DNA Purification Midi Kit 56700-NB 20 preps 437 €   DETAILS
Urine Cell-Free Circulating DNA Purification Maxi Kit 56800-NB 10 preps 332 €   DETAILS

RNA Purification Kits

Plasma/Serum RNA Purification Mini Kit (Spin Columns), 50 - 200 ul sample input 55000-NB 50 preps 431 €   DETAILS
Plasma/Serum RNA Purification Midi Kit (Spin Columns), 250 ul - 1.5 ml sample input 56100-NB 20 preps 427 €   DETAILS
Plasma/Serum RNA Purification Maxi Kit (Spin Columns), 2 - 5 ml sample input 56200-NB 10 preps 340 €   DETAILS
Urine Cell-Free Circulating RNA Purification Mini Kit 56900-NB 50 preps 431 €   DETAILS
Urine Cell-Free Circulating RNA Purification Midi Kit 57000-NB 20 preps 427 €   DETAILS
Urine Cell-Free Circulating RNA Purification Maxi Kit 57100-NB 10 preps 340 €   DETAILS

Sequential RNA DNA Isolation Kits

Plasma/Serum Circulating and Exosomal RNA and DNA Purification Kit (Spin Columns) Mini, 10 - 200 ul sample input 55200-NB 50 preps 586 €   DETAILS

Total Nucleic Acid Purification Kits

Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit 56300-NB 50 preps 356 €   DETAILS
Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit 56400-NB 20 preps 393 €   DETAILS
Plasma/Serum Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit 56500-NB 10 preps 294 €   DETAILS
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Mini Kit 59900-NB 50 preps 340 €   DETAILS
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Midi Kit 60000-NB 20 preps 402 €   DETAILS
Urine Cell-Free Circulating and Viral Nucleic Acid Purification Maxi Kit 60100-NB 10 preps 298 €   DETAILS
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