The non-enzymatic fragmentation library kits are available as ultra low input DNA library kit or amplification-free kit.
Many next generation DNA sequencing applications or sample types require the construction of PCR-amplified DNA fragment libraries. (E.g. whole genome sequencing or resequencing from limiting genomic DNA amounts or FFPE and cell-free DNA samples, exome sequencing, ChIP-seq, etc.). To get the highest quality data for these applications and sample types, you need a DNA library preparation kit with the highest efficiency adaptor ligation followed by unbiased PCR amplification to produce the most complex, unbiased libraries possible. These high quality libraries produce the high quality sequencing data with uniform depth and minimal zero coverage needed to drive your NGS experiments forward.
The NxSeq™ UltraLow DNA Library Kits and NxSeq Indexing Kits allow you to build high quality DNA fragment libraries from extremely low DNA input amounts – as low as 50 pg depending on sample type and Illumina sequencer used. If you have more DNA, no problem; you can use as much as 100 ng of input DNA with this system.
To generate these high quality libraries, each step of the protocol was optimised to ensure peak performance on Illumina sequencers. The kit contains an improved master mix that performs better by decreasing bias across high AT-rich genomic regions. Not only will you produce high quality libraries, but also once you have your fragmented DNA, library prep is quick and easy - about three total hours.
You’re doing next gen sequencing for a reason; you want big data! To get there, you know you need the best fragment libraries possible to get the most information out of each sample. So why spend your time, resources and expensive sequencing reagents on anything but the best fragment DNA libraries?
The NxSeq™ AmpFREE Low DNA Library Kit allows you to build the best fragment DNA libraries possible. Each step of the protocol was optimised to ensure peak performance on Illumina sequencers. In addition, these kits require only 75 ng of sheared DNA input, and produce libraries in about 2 hours using a streamlined, easy to follow protocol.