TetroScript Reverse Transcriptase

TetroScript Reverse Transcriptase is a high concentration, robust thermostable MMLV-RT, designed to reduce RNase H activity and increase thermal stability

Product Description

Traditional Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is not thermostable and can only maintain its enzymatic activity at relatively low temperatures (up to 50°C). However for cDNA synthesis, a higher reaction temperature is desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimizes nonspecific primer binding.

The TetroScript Reverse Transcriptase has higher thermal stability and reduced RNase H activity. The enzyme can be used to synthesize first-strand cDNA at temperatures up to 60°C which improves the cDNA yield from difficult RNA targets, like GC-rich target RNAs and RNA with high secondary structure, such as viral RNA sequences, that require higher temperature to denature strong secondary structures.

Product Highlights

• High thermostability - reverse transcriptase activity up to 60°C, ideal for RNA with high secondary structure such
  as viral genomes
• Higher enzyme efficiency and sensitivity - reliable quantification of low abundance targets and scarce samples
• Low glycerol - formulation compatible with lyophilization and air-drying applications
• Fast - delivers reproducible, multiplex accurate RT-qPCR assay results in as little as 30 minutes

Comparison of thermostability 

Performance of Meridian’s Thermostabe Reverse Transcriptase (red), standard MMLV-RT (grey) and SuperScript III thermostable reverse transcriptase (Thermo, black) after pre-incubation at 40°C to 70°C for 10 mins, in a multiplex one-step RT-qPCR assay. ∆ct values were calculated against the ct value produced by the same enzyme stored at -20 °C. The data illustrates the increased thermal stability of Meridian's MMLV-RT when compared to other MMLV-RT enzymes and its ability to efficiently synthesize cDNA at temperatures up to 60°C.