Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line

The Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line was derived from embryos of a pregnant mouse heterozygous for the bcl-x gene (bcl-x+/−). After immortalization by transfection of the SV40 large T antigen, the mouse embryonic fibroblasts were transfected with mutant Bcl-x_L cDNA. This stable cell line is able to express B-cell lymphoma-extra-large (Bcl-x_L) localized in the endoplasmic reticulum (ER). Bcl-x_L is a major regulator of cellular apoptotic signalling by expressing Bcl-2 proteins. The Stable Bcl-xL Mouse Embryonic Fibroblast (ER) Cell Line is a useful model in studying apoptosis regulation of tissue differentiation and homeostasis focusing on the specific activities of Bcl-x_L at the endoplasmic reticulum as well as how Bcl-x_L functions in the regulation of Ca²⁺ signalling. This cell line is also useful in screening and development of new therapeutic strategies for cancer and other diseases.