OxiSelect N-epsilon-(carboxymethyl) lysine (CML) Competitive ELISA Kit
| Specifications | |
|---|---|
| Sample Type: | Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples |
| Detection Method: | Colorimetric |
Product Description
- Detect CML as low as 50 ng/mL from a variety of samples
- CML-modified BSA included as standard
- Compatible with cell lysates, serum, plasma, purified proteins, and other protein-containing samples
The OxiSelect™ Methylglyoxal (MG) ELISA Kit is an enzyme immunoassay developed for rapid detection and quantitation of MG-H1 (methyl-glyoxal-hydro-imidazolone) protein adducts. The quantity of MG adduct in protein samples is determined by comparing its absorbance with that of a known MG-BSA standard curve. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown protein samples.
Assay Principle:
First, a CML conjugate is coated on the ELISA plate. The unknown CML protein samples or CML-BSA standards are then added to the CML conjugate preabsorbed plate. After a brief incubation, the anti-CML monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The content of CML protein adducts in unknown samples is determined by comparison with the predetermined CML-BSA standard curve.
Product Citations
- Dunmore SJ, et al. (2018) Evidence That Differences in Fructosamine-3-Kinase Activity May Be Associated With the Glycation Gap in Human Diabetes. Diabetes. doi: 10.2337/db17-0441.
- Cannizzaro L, et al. (2017) Regulatory landscape of AGE-RAGE-oxidative stress axis and its modulation by PPARγ activation in high fructose diet-induced metabolic syndrome. Nutr Metab (Lond). doi: 10.1186/s12986-016-0149-z.
- Svrckova M, et al. (2017) Na+/K+-ATPase interaction with methylglyoxal as reactive metabolic side product. Free Radic Biol Med. doi: 10.1016/j.freeradbiomed.2017.03.024.
- Yu Y, et al. (2017) Advanced glycation end products receptor RAGE controls myocardial dysfunction and oxidative stress in high-fat fed mice by sustaining mitochondrial dynamics and autophagy-lysosome pathway. Free Radic Biol Med. doi: 10.1016/j.freeradbiomed.2017.08.012.
- Chao, K.C. et al. (2016) Effects of ferric citrate supplementation on advanced glycation end products in a rat model of streptozotocin/nicotinamide-induced diabetes. Mol Nutr. doi:10.1002/mnfr.201600753.
- Catalog Number
STA-816-CB - Supplier
Cell Biolabs - Size
- Shipping
Blue Ice
