mGFP Expressing Osteoclastogenic Stable RAW264.7 Cell Line (H10)

Specifications
Cell Category:Stable Cell Lines
Organism:Mouse (M. musculus)
Tissue of Origin:Ascites
Morphology:Monocyte/macrophage
Culture Properties:Adherent
Pathology:Abelson murine leukemia virus-induced tumor

Propagation

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture.PriGrow III (TM003) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note:Regular subculturing is essential; avoid media colour change to yellow or cell overgrowth. Split cells at 60-70% confluency;complete confluence should be avoided.

Additional Information

The H10 cell line is a pre-osteoclast cell line established from RAW264.7 macrophages. This cell line demonstrates a high potential for RANKL-induced osteoclastogenesis. This cell lines offer a model to study the molecular processes governing the formation and function of osteoclasts, which are essential for bone resorption and remodeling.Additional cell lines from this panel:Cat. T3991 - mGFP Expressing Non-Osteoclastogenic Stable RAW264.7 Cell Line (C8)

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Genetically Modified Organism (GVO)

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Maria Schröder

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