Lyo-Ready qPCR Mix

For developing room temperature, lyophilized, multiplex qPCR diagnostic tests

Product Description

Lyo-Ready™ qPCR master mix is a glycerol-free formulation that contains Taq polymerase, reaction buffer, dNTP, MgCl2, and lyo-excipients. It is ideal for developing room temperature, lyophilized, multiplex qPCR diagnostic tests and is suited for high-throughput, automated platforms requiring milli-second rehydration times. Please refer to the Lyophilization & Post-Lyophilization User Guideline for optimal usage.

Molecular diagnostic tests are progressively moving towards lyophilization. There are several advantages for this, including room temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume. In order to be compatible with drying, however, enzyme preparations must be glycerol-free and include specialized excipients that preserve the mixture as it is exposed to freezing, vacuum, and dehydration. An ideal lyophilization formulation, for example, should stabilize an enzyme in a freeze-dried format and allow very fast rehydration and reactivation of the enzyme preparations without impacting its performance post rehydration. Can also be used wet and/or with purified nucleic acid.