Lyo-Ready Genotyping Direct qPCR Urine, 4X

Reliable genotyping directly from challenging urine samples

Product Description

Meridian’s Lyo-Ready Genotyping Direct qPCR Urine is a 4x inhibitor-resistant mix designed for highly specific and sensitive amplification of genotyping sequence variants. It can be used in the presence of PCR inhibitors found in crude urine samples, to give exceptional cluster resolution with clear allele discrimination, even with challenging SNP targets. It can also be used in a liquid or lyophilized format to create ambient-temperature stable assays, making it ideal for point-of-care (POC) devices.

For more details please see user manual above and the Lyophilization & Post-Lyophilization User Guideline.

Product Highlights

• Ultra-sensitive detection down to less than 1 copy
• Suitable for crude human urine samples
• Tight fluorescence clusters with clear allele discrimination, perfect for difficult SNPs
• Can be used as a liquid or lyophilized, ideal for POC testing
  
  

SNPs (single nucleotide polymorphisms) or point mutations are the most common types of genetic variation and comprise the major part of phenotype diversity between individuals. SNPs can be responsible for both resistance or susceptibility to a certain disease and once individual SNPs have been identified by sequencing, they can be used for pharmacogenetics, in evaluating and predicting a patient’s response to treatment and risk of adverse events, or for diagnostics, such as in cancer screening. Clinical screening of SNPs requires large-scale multiplexed genotyping, which traditionally relies on DNA extraction from complex samples such as blood, to achieve sensitive DNA amplification. However, blood collection is invasive and requires a professional, urine on the other hand is an extremely non-invasive process, that is safe, less expensive and easy to do at home, making it ideal for large population or frequent sampling, particularly when children or people that will not comply with blood collections.

Supporting Data

Fig.1. Detection of bladder cancer-associated PAI1 mutation (SNP accession number: rs7242) , in presence of 30% pooled human urine with A/ Lyo-Ready Genotyping Direct qPCR Urine Mix, B/ Roche Kapa Probe Force, C/ ThermoFisher TaqPath™ and D/ Qiagen Type-it Fast SNP Probe PCR Kits. Homozygous samples for allele T (red) and allele G (blue) and heterozygous samples for allele T/G (green) were compared with a NTC (black) and x for undetermined. The results illustrate ability of Lyo-Ready Genotyping Direct qPCR Urine to form tight clustering and so accurate allelic discrimination in the presence of 30% crude urine unlike the other mixes.