Lyo-Ready Genotyping Direct qPCR Stool, 4X

Reliable genotyping directly from challenging stool samples

Product Description

Meridian’s new Lyo-Ready Genotyping Direct qPCR Stool is a mix that is designed for fast, precise, and reproducible allelic discrimination and cluster separation. Its advanced formulation enables highly sensitive amplification of SNPs and other point mutations from stool samples and overcomes inhibitors such as bile salts that can damage DNA and inhibit PCR reactions. It can also be used in a wet format or lyophilized to create ambient-temperature stable assays, making it ideal for point-of-care (POC) devices.

For more details please see user manual above and the Lyophilization & Post-Lyophilization User Guideline.

Product Highlights

• Ultra-sensitive detection down to less than 1 copy
• Suitable for crude human stool samples
• Tight fluorescence clusters with clear allele discrimination, perfect for difficult SNPs
• Can be used as a liquid or lyophilized, ideal for POC testing
  
  

SNPs (single nucleotide polymorphisms) or point mutations are the most common types of genetic variation and comprise the major part of phenotype diversity between individuals. SNPs can be responsible for both resistance or susceptibility to a certain disease and once individual SNPs have been identified by sequencing, they can be used for pharmacogenetics, in evaluating and predicting a patient’s response to treatment and risk of adverse events, or for diagnostics, such as in cancer screening. Clinical screening of SNPs requires large-scale multiplexed genotyping, which traditionally relies on DNA extraction from complex samples such as blood, to achieve sensitive DNA amplification. However, blood collection is invasive and requires a professional, stool on the other hand is non-invasive, safe, less expensive and easy to do at home, making it ideal for POC and frequent sampling.

Supporting Data

Fig.1. Detection of colorectal cancer-associated p53 mutation (SNP accession number: rs1042522) was performed using A) Lyo-Ready Genotyping Direct qPCR Stool, B) Kapa Probe Force, C) TaqPath™ and D) Type-it Fast Kits, in presence of 20 mg/mL human stool. Homozygous Allele C (red) and allele G (blue) and heterozygous samples for allele C/G (green) were compared with a NTC (black) and x for undetermined. The results illustrate the ability of Lyo-Ready Genotyping Direct qPCR Stool to form tight clusters and to achieve accurate allelic discrimination of clinically relevant mutations directly from crude human stool samples.