Lyo-Ready Genotyping Direct qPCR Blood, 4x

Reliable genotyping directly from challenging blood samples

Product Description

Meridian's Lyo-Ready Genotyping Direct qPCR Blood is an inhibitor-resistant mix designed for highly specific and sensitive amplification of genotyping sequence variants. It can be used in the presence of PCR inhibitors found in blood, serum, or plasma and provides exceptional cluster resolution and clear allele discrimination, even with challenging SNP targets. It can also be used in liquid or lyophilized formats to create stable, ambient-temperature assays, making it ideal for point-of-care devices.

For more details please see user manual above and the Lyophilization & Post-Lyophilization User Guideline.

Product Highlights

• Ultra-sensitive detection down to less than 1 copy
• Suitable for whole blood, serum, plasma
• Tight fluorescence clusters with clear allele discrimination, perfect for difficult SNPs
• Can be used as a liquid or lyophilized, ideal for POC testing
  
  

SNPs (single nucleotide polymorphisms) or point mutations are the most common types of genetic variation and comprise the major part of phenotype diversity between individuals. SNPs can be responsible for both resistance or susceptibility to a certain disease and once individual SNPs have been identified by sequencing, they can be used for pharmacogenetics, in evaluating and predicting a patient’s response to treatment and risk of adverse events, or for diagnostics, such as in cystic fibrosis screening. Clinical screening of SNPs requires large-scale multiplexed genotyping, which traditionally relies on DNA extraction from complex samples such as blood, to achieve sensitive DNA amplification. However, blood contains several PCR inhibitors that can inhibit the reaction or reduce a reaction’s efficiency.

Supporting Data

Fig.1. 10% K2-EDTA whole human blood was tested with A/ Lyo-Ready™ Genotyping Direct qPCR Blood Mix, B/ Roche Kapa Probe Force, C/ ThermoFisher TaqPath™ and D/ Qiagen Type-it Fast SNP Probe PCR Kits, using EBV targets. Homozygous samples for allele A (red) and allele C (blue) and heterozygous samples for allele A/C (green) were compared with a NTC (black) and x for undetermined. The results illustrate ability of Lyo-Ready™ Genotyping Direct qPCR Blood to form tight clustering and so accurate allelic discrimination in the presence of whole blood unlike the other mixes.