Lyo-Ready dUTP 1-Step RT-qPCR Mix

Create RT-qPCR diagnostic tests for detecting RNA viruses at very low levels

Product Description

The Lyo-Ready dUTP 1-Step RT-qPCR Mix is a 2x RT-qPCR master mix and can be used for the development of RNA based molecular diagnostic tests, it contains a separate lyo-compatible MMLV-RT and enzyme dilution buffer. The dTTP in the dNTPs has been substituted for dUTP, so that Uracil DNA Glycosylase (UDGase) (MDX054) can be added to remove background PCR product contamination. This makes Lyo-Ready dUTP 1-Step RT-qPCR Mix ideal for developing highly sensitive RT-qPCR diagnostic tests, such as for detecting RNA viruses at very low levels.

• Very fast reaction kinetics – ideal for multiplex one-step RT-qPCR assays and low-copy number targets
• Glycerol-free mixes are pre-formulated with a specialized blend of lyo-excipients, compatible with lyophilization into beads or cakes
• Mixes can be lyophilized to create fast, highly reproducible, ambient temperature stable molecular diagnostic tests
• Prevents carry-over qPCR contamination
  
  

Molecular diagnostic tests are progressively moving towards lyophilized formats. There are several advantages for this, including ambient temperature shipping and storage, extended shelf-life, reduced operating steps and potential errors and increased flexibility in sample volume. In order to be compatible with drying, however, enzyme preparations must be glycerol-free and include specialized excipients that preserve the mixture as it is exposed to various conditions including freezing, vacuum, and dehydration.

Meridians Lyo-Ready dUTP 1-Step RT-qPCR Mix is a glycerol-free mix, ideal for developing ambient temperature, lyophilized, multiplex qPCR and RT-qPCR molecular diagnostic tests and are suited for high-throughput, automated platforms.

Performance Data

Fig.1. High efficiency and sensitivity from both DNA (red) and RNA (blue) templates. Amplification of both Rotavirus A (dsRNA) and Adenovirus (dsDNA) in a single multiplexed RT-qPCR assay, from inactivated crude viral lysates. The result illustrates that both the DNA and RNA viruses were amplified with high efficiency and sensitivity, demonstrating the ability of Lyo-Ready 1-Step RT-qPCR Virus Mix to detect low-copy number RNA and DNA targets simultaneously from a single sample.