Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag (MALS verified)
| Specifications | |
|---|---|
| Category: | Enzymes |
| Molecular Weight: | 14.5 kDa & 13.8 kDa & 25.8 kDa |
| Tag: | N-Strep II | N-Strep II | N-10×His |
| Label / Conjugate: | N/A |
| Source: | Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag (ELL-H5595) is expressed from Baculovirus-Insect cells. It contains AA Asp 2 - Gln 118 (Elongin B) & Asp 2 - Cys 112 (Elongin C) & Pro 2 - Asp 213 (VHL) (Accession # Q15370-1 (Elongin B) & Q15369-1 (Elongin C) & P40337-2 (VHL)). |
| Purity: | 95% |
| Endotoxin Level: | 1.0 EU per μg |
| Format: | Liquid |
Sequence Information
Asp 2 - Gln 118 (Elongin B) & Asp 2 - Cys 112 (Elongin C) & Pro 2 - Asp 213 (VHL)
Background
Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites. The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. In this function, ELOB/ELOC serves as an adapter between substrate recognition proteins and either Cullin-2/Rbx1 (in VHL-box E3 Ubiquitin ligases) or Cullin-5/Rbx2 (in SOCS-box E3 Ubiquitin ligases). VHL is involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases.
Price
481,00 €
