Hamster (CHO) Legumain (LGM) ELISA

Legumain was ranked 6th out of the 30 most abundant CHO host cell proteins (HCPs) in one study of host cell proteins in batch and fed-batch CHO cultures. Legumain is a lysosomal protease that forms acidic charge variants by removing a functional group from asparagine residues (asparagine deamidation) of monoclonal antibodies, negatively impacting therapeutic antibody quality. (Park, J.H. et al. (2017). Proteomic Analysis of Host Cell Protein Dynamics in the Culture Supernatants of Antibody-Producing CHO Cells. Nature: Scientific Reports. 7:44246. DOI: 10.1038/srep44246)