Fatty Acid Amide Hydrolase (FAAH) Activity Fluorometric Assay Kit

Fatty acid amide hydrolase (FAAH) is a membrane binding protein belonging to the serine hydrolase family. FAAH hydrolyzes many important endogenous fatty acid amides, including endocannabinoid (AEA) and palmitoyl glycolamide (PEA). FAAH plays a key role in controlling cannabinoid signaling by hydrolyzing 2arachidenol glycerol in AEA and certain tissues. Detection principle: FAAH specifically cut the substrate, the fluorophores emit fluorescence, and detect fluorescence at the excitation wavelength of 340 nm and the emission wavelength of 440 nm. Because FAAH is a serine protein, FAAHspecific inhibitors are used in the kit to inhibit enzyme activity and exclude the effects of non-specific enzyme activity.