Everspark 1.0 Super-resolution microscopy mounting buffer

For multicolor, long-term and stable fluorescence imaging

Product Description

Prepare your samples one day, and image them every day for weeks.

Compatible Fluorophores

Yellow to far-red. Validated fluorophores include  JF 549, DL 550, CF 555, CF 568, AF 568,JF 646, AF 647, CF 647, Atto 647N, DL 650, CF 680 & Cy5 

Features

Multicolor imaging: +

Blinking stability (after mounting): up to 2 months

Blinking events: +

Related Product

Everspark 2.0 Super-resolution microscopy mounting buffer with different compatible fluorophores (green, yellow & far-red) and improved imaging and blinking features.

Applications

• Use this hassle-free buffer to decouple the preparation of your samples and their imaging with dSTORM
• Prepare your samples in your lab, and image them in your core facility 3 to 4 weeks later
• Use different colors either at the same time or one after the other

How it Works

Use with a glass-bottom dish

Use with a depression slide

Supporting Data

Figure 1. Long-term imaging with Everspark 1.0. Left: Two centrosomes imaged the same Day (D0) and 7 days after mounting (D7) on the same slide stored in the dark at 4°C.  The typical 450 nm donut-like structure is visualised using a colour-coded scale encoded with the  IGOR software, where each point appears as a function of its localisation precision (5 to 60 nm; inverted rainbow colour scale). Labeling: Distal-appendages detected by immunofluorescence with AF647 in RPE-1 cells. 
Right: The number of blinking events per centrosome and the median of  the localization precision in nm are presented for each serie of 50,000 images recorded at D0 and D7 (left). 
Credits: Camille Fourneaux & Karine Monier, CRCL, Lyon

Figure 2. Donut-like structure of in-cellulo mature centrosome reconstructed after dSTORM imaging in Everspark 1.0 buffer.
Each point is represented by its centroid (purple points) and its gaussian width (white). Intensity is displayed on the right with the measurement from peak to peak, in agreement with the size of the distal appendage crone. Labeling: Distal appendages detected by immunofluorescence with AF647 in RPE-1 cells. Credit: @Corentin Rousset, Karine Monier, CRCL, Lyon