Enhanced Primary Human Liver Sinusoidal Endothelial Cells

Specifications
Cell Category:Primary Cells
Organism:Human
Tissue of Origin:Liver
Morphology:Endothelial-like
Culture Properties:Adherent

Propagation

Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. ready-to-use Human Liver Sinusoidal Endothelial Cell Thawing Medium (TM111) and the Human Liver Sinusoidal Endothelial Cell Media Kit (TM112) which comes with the Human Liver Sinusoidal Endothelial Cell Basal Medium, and Human Liver Sinusoidal Endothelial Cell Supplement Mix are required for growth of the cells, 37.0°C, 5% CO₂.

Additional Information

Enhanced Primary Human Liver Sinusoidal Endothelial Cells are expanded primary cells that retain the properties of primary liver sinusoidal endothelial cells which allows for a reliable in vitro system. These cells are ready-to-use for applications in research and development, such as viral infections, screening, co-cultures, transient transfection, and for toxicity studies. The cells offer a unique ability to be kept in longer term culture when compared to standard primary liver sinusoidal endothelial cells which does not proliferate well in vitro.

The cells are offered ready-to-use after thawing. Recommend to only passage the cells 1-2 more times, as more passages may see phenotypical changes and expression of senescence genes.

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Researchers at for-profit organizations and corporations, please contact us for pricing of this item.

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Please contact:

Maria Schröder

Tel. +49 (0) 6221 71415 16

info@biocat.com

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