CytoSelect™ Cell Viability and Cytotoxicity Assay

Product Description

• Detect live and dead cells by microscopy, plate reader, or flow cytometry
• Both live and dead cells may be quantified on a fluorescence plate reader; live cells may also be quantified on a standard colorimetric (ELISA) plate reader

Cell Biolabs’ CytoSelect™ Cell Viability and Cytotoxicity Assay Kit provide a colorimetric and fluorometric format for measuring and monitoring cell viability. The kit contains MTT reagent, Calcein AM, and Ethidium Homodimer. Detergent and Lysis Buffer are provided for extracting the MTT reagent or the Calcein AM/EthD-1 from cell samples. Saponin, a cell death initiator, is also included as a control. The kit is suitable for use with light and fluorescence microscopes, colorimetric and fluorometric multiwell plate scanners, flow cytometers, and other colorimetric or fluorometric detection systems. The kit contains sufficient reagents for the evaluation of 96 assays in a 96-well plate, or 24 assays in a 24-well plate. Cells can then be treated with compounds or agents that affect viability. Live cells are detected with the MTT reagent as well as the Calcein AM. Dead cells are detected with the EthD-1 reagent. Finally, cell viability/cytotoxicity is determined using the colorimetric and fluorometric detection reagents. An increase in cell viability is accompanied by cell growth, while a decrease in cell viability can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. The kit does not react with bacteria or yeast.

Human Foreskin Fibroblast BJ-TERT Cell Viability. BJ-TERT cells were seeded at 50,000 cells/well and allowed to culture for 24 hours. Cells were then treated with and without Saponin. Cell samples were then treated with MTT, Calcein AM or EthD-1.