CoviDrop SARS-CoV-2 Spike-ACE2 Binding Activity/Inhibition Assay Kit

Product Description

• Colorimetric assay with easy-to-follow steps
• Extremely low background signals
• Simple, accurate, reliable, and consistent assay
• For plasma, serum, swab samples, other body fluids, and purified proteins
• Activity detection from as low as 0.1 ng of ACE2
• Quantitative assay
• Strip-well microplate format for manual or high throughput analysis

The CoviDrop™ SARS-CoV-2 Spike-ACE2 Binding Activity/Inhibition Assay Kit is a complete set of optimized buffers and reagents designed for measuring SARS-CoV-2-ACE2 binding activity and inhibition using various human samples so that the functional ACE2 binding level from different cells/tissues in different individuals can be identified. The samples can be used immediately or stored at proper conditions for future use.

Fig.1. SARS-Cov-2 binding to ACE2 and entry into cells

Principles and Procedure

Fig.2. Schematic procedure of the CoviDrop SARS-CoV-2 Spike-ACE2 Binding Activity/Inhibition Assay Kit

In this assay, a SARS-CoV-2 spike protein is stably pre-coated onto microplate wells. ACE2 contained in the sample is captured by the coated spike protein. The amount of the captured ACE2, which is proportional to ACE2 binding activity, is then recognized by anti-ACE2 antibody and measured through an ELISA-like reaction by reading the absorbance in a microplate spectrophotometer at a wavelength of 450 nm. The binding activity of ACE2 is proportional to the optical density intensity measured.

Starting Materials

Input materials can be cell/tissue extracts, serum, plasma, swab samples, and various body fluids.