Cas9 Expressig U2OS Cell Line

Specifications
Cell Category:Stable Cell Lines
Organism:Human (H. sapiens)
Tissue of Origin:Bone
Morphology:Epithelial
Culture Properties:Adherent
Marker:Amelogenin: X
Donor Age:15
Donor Gender:Female
Donor Ethnicity:White
Pathology:Osteosarcoma

Propagation

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. M199 Medium (TM502) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂. 0.4 µg/ml Puromycin (G264) for selection. Note: Selection drugs should be added to the culture medium after the first passage to ensure cells have recovered from freeze-thaw conditions.

Additional Information

This U2OS cell line stably expresses spCas9.

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Genetically Modified Organism (GVO)

See General Terms.

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Please contact:

Maria Schröder

Tel. +49 (0) 6221 71415 16

info@biocat.com

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