axoCells Human iPSC-derived Mature Microglia (male donor)

Product Description

axoCells Human iPSC-Derived Microglia (≥1 million cells) for neuroscience research. Microglia made from iPSCs generated from a 40-50-year-old male donor's fibroblasts.

• Assay ready in just 7 days 
• Express the key markers including IBA-1, TMEM119 and P2RY12
• Demonstrate robust functional activity, measured by cytokine release, phagocytosis and chemotaxis

With axoCells, you can rely on physiologically relevant microglia that express the three key markers (IBA1, TMEM119, and P2RY12) and demonstrate robust functional activity, measured by cytokine release, phagocytosis, and chemotaxis. With Axol's microglia, you can build robust mono- and co-culture models to investigate neurological diseases and drug discovery.

Supporting Data

Phenotypic characterization: Morphology

Fig.1: axoCells iPSC-derived microglia demonstrate appropriate morphology, with variable phenotypes demonstrating different populations of microglial subtype. Cryopreserved axoCells microglia (ax0664) were thawed and matured for 7 days on Surebond XF + concanavalin A.

Fig.2: axoCells microglia have been extensively characterized for expression the key markers Iba1, TMEM119, CX3CR1 and P2RY12. axoCells iPSC-derived microglia were thawed and matured for 7 days. Cells were fixed and stained for the relevant markers and imaged using a Leica microscope. Key microglia markers Iba1, TMEM119, CX3CR1 and P2RY12 were used as standard quality control. Red: marker of interest, Blue: DAPI (neuronal marker).

Fig.3: Flow cytometry QC for fresh macrophage progenitors (monocytes). Progenitor cells from all batches undergo QC for a standard panel of markers.

As part a rigorous QC, flow cytometry is included as standard to demonstrate committal down the myeloid lineage, with SSEA4 (a marker of immaturity) as a negative control.