Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3A)

PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS + 1% L-glutamine (G275) + 400 ng/ml hydrocortisone (TM066) + 20 ng/ml rhEGF (Z100139) + 5 ug/ml recombinant human Insulin (Z101065) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: re-suspend cells well at each stage (thawing, post-centrifugation etc.) as cells have a tendency to clump. Freeze-thaw viability for this cell line varies. For best results, seed thawed cells, collect floating cells the next day, centrifuge and re-plate into a new flask. 

The UM-HMC series, encompassing UM-HMC-1, UM-HMC-3B, and UM-HMC-3A, are pioneering cell lines derived from human salivary mucoepidermoid carcinomas, pivotal for advancing research in oncology and cellular biology. Originating from a primary tumor (UM-HMC-1) from a male patient, and recurrence and lymph node metastasis from a female patient (UM-HMC-3A and UM-HMC-3B, respectively), these cell lines have been instrumental in characterizing the tumorigenic potential and cancer stem cell properties within salivary gland carcinomas. Studies utilizing these cell lines have shed light on critical aspects of cancer biology, including the role of ALDH/CD44 in identifying tumorigenic cancer stem cells, the necessity of mTOR signaling for the survival of these cancer stem cells, and the influence of p53 in regulating Bmi-1-driven self-renewal and stemness. As such, the UM-HMC series represents a valuable resource for researchers aiming to explore the molecular mechanisms underlying salivary gland cancer progression, metastasis, and treatment resistance, thereby offering potential pathways for developing targeted therapies.Complete list of cell lines from this panel: Cat. T8160 - Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3A)Cat. T8161 - Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-3B)Cat. T8173 - Human Mucoepidermoid Carcinoma Cell Line (UM-HMC-1)